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Literature summary extracted from

  • Day, I.J.; Maeda, K.; Paisley, H.J.; Mok, K.H.; Hore, P.J.
    Refolding of ribonuclease A monitored by real-time photo-CIDNP NMR spectroscopy (2009), J. Biomol. NMR, 44, 77-86.
    View publication on PubMed

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.6.1.18 guanidine-HCl real-time photo-CIDNP spectra from guanidine-HCl refolding experiments Bos taurus
4.6.1.18 urea photo-CIDNP NMR spectroscopy for monitoring of the real-time refolding of ribonuclease A following dilution from a high concentration of urea denaturant Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Bos taurus P61823
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-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.6.1.18 photo-CIDNP NMR spectroscopy study for monitoring of the real-time refolding of ribonuclease A following dilution from a high concentration of urea or guanidine-HCl denaturant, two distinct kinetic processes are apparent, a faster step with time constant of 4-8 s (4.2 s in urea and 7.3/7.6 s in guanidine-HCl) and a slower one with time constant 16-24 s (16.6 s in urea and 24.0 s in guanidine-HCl). The latter is attributed to the cis-trans isomerization of Pro 93 and is responsible for the slow disappearance of the signal from Tyr 92 in the folding intermediate observed in the guanidine-HCl experiment Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.18 commercial preparation
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 Bos taurus
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Synonyms

EC Number Synonyms Comment Organism
4.6.1.18 pancreatic ribonuclease A
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 Bos taurus
4.6.1.18 RNase A
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 Bos taurus